An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading
Joint Authors
Sun, Shan
Jones, T. D.
Kefi, A.
Cho, M.
Alapati, S. B.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
HyStem-C™ is a commercially available injectable hydrogel composed of polyethylene glycol diacrylate (PEGDA), hyaluronan (HA), and gelatin (Gn).
These components can be mechanically tuned to enhance cell viability and spreading.
Methods.
The concentration of PEGDA with an added disulfide bond (PEGSSDA) was varied from 0.5 to 8.0% (w/v) to determine the optimal concentration for injectable clinical application.
We evaluated the cell viability of human dental pulp stem cells (hDPSCs) embedded in 2% (w/v) PEGSSDA-HA-Gn hydrogels.
Volume ratios of HA : Gn from 100 : 0 to 25 : 75 were varied to encourage hDPSC spreading.
Fibronectin (Fn) was added to our model to determine the effect of extracellular matrix protein concentration on hDPSC behavior.
Results.
Our preliminary data suggests that the hydrogel gelation time decreased as the PEGSSDA cross-linker concentration increased.
The PEGSSDA-HA-Gn was biocompatible with hDPSCs, and increased ratios of HA : Gn enhanced cell viability for 14 days.
Additionally, cell proliferation with added fibronectin increased significantly over time at concentrations of 1.0 and 10.0 μg/mL in PEGDA-HA-Gn hydrogels, while cell spreading significantly increased at Fn concentrations of 0.1 μg/mL.
Conclusions.
This study demonstrates that PEG-based injectable hydrogels maintain hDPSC viability and facilitate cell spreading, mainly in the presence of extracellular matrix (ECM) proteins.
American Psychological Association (APA)
Jones, T. D.& Kefi, A.& Sun, Shan& Cho, M.& Alapati, S. B.. 2016. An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading. Advances in Medicine،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1095332
Modern Language Association (MLA)
Jones, T. D.…[et al.]. An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading. Advances in Medicine No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1095332
American Medical Association (AMA)
Jones, T. D.& Kefi, A.& Sun, Shan& Cho, M.& Alapati, S. B.. An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading. Advances in Medicine. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1095332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1095332