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Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and BaseAlternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine
Joint Authors
Ishino, Yoshizumi
Abellón-Ruiz, Javier
Ishino, Sonoko
Connolly, Bernard A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In Archaea repair of uracil and hypoxanthine, which arise by deamination of cytosine and adenine, respectively, is initiated by three enzymes: Uracil-DNA-glycosylase (UDG, which recognises uracil); Endonuclease V (EndoV, which recognises hypoxanthine); and Endonuclease Q (EndoQ), (which recognises both uracil and hypoxanthine).
Two archaeal DNA polymerases, Pol-B and Pol-D, are inhibited by deaminated bases in template strands, a feature unique to this domain.
Thus the three repair enzymes and the two polymerases show overlapping specificity for uracil and hypoxanthine.
Here it is demonstrated that binding of Pol-D to primer-templates containing deaminated bases inhibits the activity of UDG, EndoV, and EndoQ.
Similarly Pol-B almost completely turns off EndoQ, extending earlier work that demonstrated that Pol-B reduces catalysis by UDG and EndoV.
Pol-B was observed to be a more potent inhibitor of the enzymes compared to Pol-D.
Although Pol-D is directly inhibited by template strand uracil, the presence of Pol-B further suppresses any residual activity of Pol-D, to near-zero levels.
The results are compatible with Pol-D acting as the replicative polymerase and Pol-B functioning primarily as a guardian preventing deaminated base-induced DNA mutations.
American Psychological Association (APA)
Abellón-Ruiz, Javier& Ishino, Sonoko& Ishino, Yoshizumi& Connolly, Bernard A.. 2016. Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and BaseAlternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine. Archaea،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1096640
Modern Language Association (MLA)
Abellón-Ruiz, Javier…[et al.]. Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and BaseAlternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine. Archaea No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1096640
American Medical Association (AMA)
Abellón-Ruiz, Javier& Ishino, Sonoko& Ishino, Yoshizumi& Connolly, Bernard A.. Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and BaseAlternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine. Archaea. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1096640
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096640