Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste
Joint Authors
Esquivel-Elizondo, Sofia
Parameswaran, Prathap
Delgado, Anca G.
Maldonado, Juan
Rittmann, Bruce E.
Krajmalnik-Brown, Rosa
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Inhibition by ammonium at concentrations above 1000 mgN/L is known to harm the methanogenesis phase of anaerobic digestion.
We anaerobically digested swine waste and achieved steady state COD-removal efficiency of around 52% with no fatty-acid or H2 accumulation.
As the anaerobic microbial community adapted to the gradual increase of total ammonia-N (NH3-N) from 890 ± 295 to 2040 ± 30 mg/L, the Bacterial and Archaeal communities became less diverse.
Phylotypes most closely related to hydrogenotrophic Methanoculleus (36.4%) and Methanobrevibacter (11.6%), along with acetoclastic Methanosaeta (29.3%), became the most abundant Archaeal sequences during acclimation.
This was accompanied by a sharp increase in the relative abundances of phylotypes most closely related to acetogens and fatty-acid producers (Clostridium, Coprococcus, and Sphaerochaeta) and syntrophic fatty-acid Bacteria (Syntrophomonas, Clostridium, Clostridiaceae species, and Cloacamonaceae species) that have metabolic capabilities for butyrate and propionate fermentation, as well as for reverse acetogenesis.
Our results provide evidence countering a prevailing theory that acetoclastic methanogens are selectively inhibited when the total ammonia-N concentration is greater than ~1000 mgN/L.
Instead, acetoclastic and hydrogenotrophic methanogens coexisted in the presence of total ammonia-N of ~2000 mgN/L by establishing syntrophic relationships with fatty-acid fermenters, as well as homoacetogens able to carry out forward and reverse acetogenesis.
American Psychological Association (APA)
Esquivel-Elizondo, Sofia& Parameswaran, Prathap& Delgado, Anca G.& Maldonado, Juan& Rittmann, Bruce E.& Krajmalnik-Brown, Rosa. 2016. Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste. Archaea،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096648
Modern Language Association (MLA)
Esquivel-Elizondo, Sofia…[et al.]. Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste. Archaea No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096648
American Medical Association (AMA)
Esquivel-Elizondo, Sofia& Parameswaran, Prathap& Delgado, Anca G.& Maldonado, Juan& Rittmann, Bruce E.& Krajmalnik-Brown, Rosa. Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste. Archaea. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096648
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096648