Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii
Joint Authors
Pei, Jihua
Yan, Jianfang
Jiang, Yi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The Lon protease is highly evolutionarily conserved.
However, little is known about Lon in the context of gut microbial communities.
A gene encoding a Lon-like protease (Lon-like-Ms) was identified and characterized from Methanobrevibacter smithii, the predominant archaeon in the human gut ecosystem.
Phylogenetic and sequence analyses showed that Lon-like-Ms and its homologs are newly identified members of the Lon family.
A recombinant form of the enzyme was purified by affinity chromatography, and its catalytic properties were examined.
Recombinant Lon-like-Ms exhibited ATPase activity and cleavage activity toward fluorogenic peptides and casein.
The peptidase activity of Lon-like-Ms relied strictly on Mg2+ (or other divalent cations) and ATP.
These results highlight a new type of Lon-like protease that differs from its bacterial counterpart.
American Psychological Association (APA)
Pei, Jihua& Yan, Jianfang& Jiang, Yi. 2016. Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii. Archaea،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1096650
Modern Language Association (MLA)
Pei, Jihua…[et al.]. Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii. Archaea No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1096650
American Medical Association (AMA)
Pei, Jihua& Yan, Jianfang& Jiang, Yi. Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii. Archaea. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1096650
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096650