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Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GON Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium
Joint Authors
Fujii, Miki
Hata, Chieri
Ukita, Munetada
Fukushima, Chie
Matsuura, Chihiro
Kawashima-Ohya, Yoshie
Tomobe, Koji
Kawashima, Tsuyoshi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The oxidation of guanine (G) to 7,8-dihydro-8-oxoguanine (GO) forms one of the major DNA lesions generated by reactive oxygen species (ROS).
The GO can be corrected by GO DNA glycosylases (Ogg), enzymes involved in base excision repair (BER).
Unrepaired GO induces mismatched base pairing with adenine (A); as a result, the mismatch causes a point mutation, from G paired with cytosine (C) to thymine (T) paired with adenine (A), during DNA replication.
Here, we report the characterization of a putative Ogg from the thermoacidophilic archaeon Thermoplasma volcanium.
The 204-amino acid sequence of the putative Ogg (TVG_RS00315) shares significant sequence homology with the DNA glycosylases of Methanocaldococcus jannaschii (MjaOgg) and Sulfolobus solfataricus (SsoOgg).
The six histidine-tagged recombinant TVG_RS00315 protein gene was expressed in Escherichia coli and purified.
The Ogg protein is thermostable, with optimal activity near a pH of 7.5 and a temperature of 60°C.
The enzyme displays DNA glycosylase, and apurinic/apyrimidinic (AP) lyase activities on GO/N (where N is A, T, G, or C) mismatch; yet it cannot eliminate U from U/G or T from T/G, as mismatch glycosylase (MIG) can.
These results indicate that TvoOgg-encoding TVG_RS00315 is a member of the Ogg2 family of T.
volcanium.
American Psychological Association (APA)
Fujii, Miki& Hata, Chieri& Ukita, Munetada& Fukushima, Chie& Matsuura, Chihiro& Kawashima-Ohya, Yoshie…[et al.]. 2016. Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GON Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium. Archaea،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096657
Modern Language Association (MLA)
Fujii, Miki…[et al.]. Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GON Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium. Archaea No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096657
American Medical Association (AMA)
Fujii, Miki& Hata, Chieri& Ukita, Munetada& Fukushima, Chie& Matsuura, Chihiro& Kawashima-Ohya, Yoshie…[et al.]. Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GON Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium. Archaea. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096657