![](/images/graphics-bg.png)
Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification
Joint Authors
Belec, L.
Mossoro-Kpinde, Christian Diamant
Mboumba Bouassa, Ralph-Sydney
Jenabian, Mohammad-Ali
Wolyec, Serge Tonen
Robin, Leman
Matta, Mathieu
Longo, Jean de Dieu
Grésenguet, Gérard
Andreoletti, Laurent
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objectives.
We evaluated the performances of Amplix real-time PCR platform developed by Biosynex (Strasbourg, France), combining automated station extraction (Amplix station 16 Dx) and real-time PCR (Amplix NG), for quantifying plasma HIV-1 RNA by lyophilized HIV-1 RNA-based Amplix reagents targeting gag and LTR, using samples from HIV-1-infected adults from Central African Republic.
Results.
Amplix real-time PCR assay showed low limit of detection (28 copies/mL), across wide dynamic range (1.4–10 log copies/mL), 100% sensitivity and 99% specificity, high reproducibility, and accuracy with mean bias < 5%.
The assay showed excellent correlations and concordance of 95.3% with the reference HIV-1 RNA load assay (Roche), with mean absolute bias of +0.097 log copies/mL by Bland-Altman analysis.
The assay was able to detect and quantify the most prevalent HIV-1 subtype strains and the majority of non-B subtypes, CRFs of HIV-1 group M, and HIV-1 groups N and O circulating in Central Africa.
The Amplix assay showed 100% sensitivity and 99.6% specificity to diagnose virological failure in clinical samples from antiretroviral drug-experienced patients.
Conclusions.
The HIV-1 RNA-based Amplix real-time PCR platform constitutes sensitive and reliable system for clinical monitoring of HIV-1 RNA load in HIV-1-infected children and adults, particularly adapted to intermediate laboratory facilities in sub-Saharan Africa.
American Psychological Association (APA)
Mossoro-Kpinde, Christian Diamant& Mboumba Bouassa, Ralph-Sydney& Jenabian, Mohammad-Ali& Wolyec, Serge Tonen& Robin, Leman& Matta, Mathieu…[et al.]. 2016. Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification. AIDS Research and Treatment،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096710
Modern Language Association (MLA)
Mossoro-Kpinde, Christian Diamant…[et al.]. Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification. AIDS Research and Treatment No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096710
American Medical Association (AMA)
Mossoro-Kpinde, Christian Diamant& Mboumba Bouassa, Ralph-Sydney& Jenabian, Mohammad-Ali& Wolyec, Serge Tonen& Robin, Leman& Matta, Mathieu…[et al.]. Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification. AIDS Research and Treatment. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096710