Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
Joint Authors
Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-15
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry.
Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells.
Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture.
While viral growth in cell culture was evidenced by the development of cytopathic effects.
The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy.
Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search.
Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein.
Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates.
Based on these findings, ALV was concluded to be associated with the present outbreak.
It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate.
American Psychological Association (APA)
Bande, Faruku& Arshad, Siti Suri& Omar, Abdul Rahman. 2016. Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken. Advances in Virology،Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1096780
Modern Language Association (MLA)
Bande, Faruku…[et al.]. Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken. Advances in Virology No. 2016 (2016), pp.1-4.
https://search.emarefa.net/detail/BIM-1096780
American Medical Association (AMA)
Bande, Faruku& Arshad, Siti Suri& Omar, Abdul Rahman. Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken. Advances in Virology. 2016. Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1096780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096780