Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368)
Joint Authors
Zhao, Dongxin
Huang, Zhong-Xian
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Zinc finger proteins are associated with hereditary diseases and cancers.
To obtain an adequate amount of zinc finger proteins for studying their properties, structure, and functions, many protein expression systems are used.
ZNF191(243-368) is a zinc finger protein and can be fused with His-tag to generate fusion proteins such as His6-ZNF191(243-368) and ZNF191(243-368)-His8.
The purification of His-tag protein using Ni-NTA resin can overcome the difficulty of ZNF191(243-368) separation caused by inclusion body formation.
The influences of His-tag on ZNF191(243-368) properties and structure were investigated using spectrographic techniques and hydrolase experiment.
Our findings suggest that insertion of a His-tag at the N-terminal or C-terminal end of ZNF191(243-368) has different effects on the protein.
Therefore, an expression system should be considered based on the properties and structure of the protein.
Furthermore, the hydrolase activity of ZNF191(243-368)-His8 has provided new insights into the design of biological functional molecules.
American Psychological Association (APA)
Zhao, Dongxin& Huang, Zhong-Xian. 2016. Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368). Bioinorganic Chemistry and Applications،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1096803
Modern Language Association (MLA)
Zhao, Dongxin& Huang, Zhong-Xian. Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368). Bioinorganic Chemistry and Applications No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1096803
American Medical Association (AMA)
Zhao, Dongxin& Huang, Zhong-Xian. Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368). Bioinorganic Chemistry and Applications. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1096803
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096803