Aberrant Expression Profile of Long Noncoding RNA in Human Sinonasal Squamous Cell Carcinoma by Microarray Analysis

Joint Authors

Meng, Ling-zhao
Fang, Ju-gao
Sun, Jing-wu
Yang, Fan
Wei, Yong-xiang

Source

BioMed Research International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-12-01

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Objectives.

This study aimed to identify aberrantly expressed long noncoding RNAs (lncRNAs) profile of sinonasal squamous cell carcinoma (SSCC) and explore their potential functions.

Methods.

We investigated lncRNA and mRNA expression in SSCC and paired adjacent noncancerous tissues obtained from 6 patients with microarrays.

Gene ontology (GO) analysis and pathway analysis were utilized to investigate the gene function.

Gene signal-network and lncRNA-mRNA network were depicted.

Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to validate 5 lncRNAs in a second set of paired SSCC and adjacent noncancerous tissues obtained from 22 additional patients.

Results.

We identified significantly differentially expressed lncRNAs (n=3146) and mRNAs (n=2208) in SSCC relative to noncancerous tissues.

The GO annotation indicated that there are some core gene products that may be attributed to the progress of SSCC.

The pathway analysis identified many pathways associated with cancer.

The results of lncRNA-mRNA network and gene signal-network implied some core lncRNAs/mRNAs might play important roles in SSCC pathogenesis.

The results of qRT-PCR showed that all of the 5 lncRNAs were differentially expressed and consistent with the microarray results.

Conclusion.

Our study is the first screening and analysis of lncRNAs expression profile in SSCC and may offer new insights into pathogenesis of this disease.

American Psychological Association (APA)

Meng, Ling-zhao& Fang, Ju-gao& Sun, Jing-wu& Yang, Fan& Wei, Yong-xiang. 2016. Aberrant Expression Profile of Long Noncoding RNA in Human Sinonasal Squamous Cell Carcinoma by Microarray Analysis. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096826

Modern Language Association (MLA)

Meng, Ling-zhao…[et al.]. Aberrant Expression Profile of Long Noncoding RNA in Human Sinonasal Squamous Cell Carcinoma by Microarray Analysis. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096826

American Medical Association (AMA)

Meng, Ling-zhao& Fang, Ju-gao& Sun, Jing-wu& Yang, Fan& Wei, Yong-xiang. Aberrant Expression Profile of Long Noncoding RNA in Human Sinonasal Squamous Cell Carcinoma by Microarray Analysis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096826

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1096826