Exposure to p,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-CEBPβ Pathway in Human Promyelocytic HL-60 Cells
Joint Authors
Calderón-Aranda, Emma S.
Torres-Avilés, Nallely A.
Albores-García, Damaris
Luna, Ana L.
Moreno-Galván, Monica
Salgado-Bustamante, Mariana
Portales-Pérez, Diana Patricia
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Dichlorodiphenyldichloroethylene (p,p′-DDE), the most persistent metabolite of dichlorodiphenyltrichloroethane (DDT), is still present in the human population.
Both are present in the bone marrow of patients with bone marrow disorders, but thus far there are no studies that assess the capability of p,p′-DDE to affect myeloid cells.
The aim of this study was to determine the effect of p,p′-DDE on promyelocytic cell differentiation and intracellular pathways related to this event.
p,p′-DDE induced morphological changes compatible with promyelocytic differentiation in a concentration-dependent manner.
The p,p′-DDE effect on Ca2+i, C/EBPβ protein levels, PKCα and p38 activation, and the role of oxidative stress or PLA2 was assayed.
Exposure to 1.9 μg/mL of p,p′-DDE increased Ca2+i, PKCα, p38, and C/EBPβ protein levels; the increase of nuclear C/EBPβ protein was dependent on p38.
PKCα phosphorylation was dependent on PLA2 and p,p′-DDE-induced oxidative stress.
p38 phosphorylation induced by p,p′-DDE was dependent on PLA2, PKC activation, and oxidative stress.
These effects of p,p′-DDE at concentrations found in human bone marrow may induce alterations in immature myeloid cells and could affect their cellular homeostasis.
In order to establish the risk from exposure to p,p′-DDE on the development of bone marrow disorders in humans, these effects deserve further study.
American Psychological Association (APA)
Torres-Avilés, Nallely A.& Albores-García, Damaris& Luna, Ana L.& Moreno-Galván, Monica& Salgado-Bustamante, Mariana& Portales-Pérez, Diana Patricia…[et al.]. 2016. Exposure to p,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-CEBPβ Pathway in Human Promyelocytic HL-60 Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096862
Modern Language Association (MLA)
Torres-Avilés, Nallely A.…[et al.]. Exposure to p,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-CEBPβ Pathway in Human Promyelocytic HL-60 Cells. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096862
American Medical Association (AMA)
Torres-Avilés, Nallely A.& Albores-García, Damaris& Luna, Ana L.& Moreno-Galván, Monica& Salgado-Bustamante, Mariana& Portales-Pérez, Diana Patricia…[et al.]. Exposure to p,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-CEBPβ Pathway in Human Promyelocytic HL-60 Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096862
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096862