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Comparison of the Effects of Dibutyl and Monobutyl Phthalates on the Steroidogenesis of Rat Immature Leydig Cells
Joint Authors
Ge, Ren-Shan
Hu, Guo-Xin
Li, Linxi
Chen, Xiaomin
Wang, Sicong
Xu, Renai
Zhu, Qiqi
Li, Xiaoheng
Wang, Mingcang
Lian, Qingquan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Dibutyl phthalate (DBP) is a widely used synthetic phthalic diester and monobutyl phthalate (MBP) is its main metabolite.
DBP can be released into the environment and potentially disrupting mammalian male reproductive endocrine system.
However, the potencies of DBP and MBP to inhibit Leydig cell steroidogenesis and their possible mechanisms are not clear.
Immature Leydig cells isolated from rats were cultured with 0.05–50 μ M DBP or MBP for 3 h in combination with testosterone synthesis regulator or intermediate.
The concentrations of 5 α -androstanediol and testosterone in the media were measured, and the mRNA levels of the androgen biosynthetic genes were detected by qPCR.
The direct actions of DBP or MBP on CYP11A1, CYP17A1, SRD5A1, and AKR1C14 activities were measured.
MBP inhibited androgen production by the immature Leydig cell at as low as 50 nM, while 50 μ M was required for DBP to suppress its androgen production.
MBP mainly downregulated Cyp11a1 and Hsd3b1 expression levels at 50 nM.
However, 50 μ M DBP downregulated Star, Hsd3b1, and Hsd17b3 expression levels and directly inhibited CYP11A1 and CYP17A1 activities.
In conclusion, DBP is metabolized to more potent inhibitor MBP that downregulated the expression levels of some androgen biosynthetic enzymes.
American Psychological Association (APA)
Li, Linxi& Chen, Xiaomin& Hu, Guo-Xin& Wang, Sicong& Xu, Renai& Zhu, Qiqi…[et al.]. 2016. Comparison of the Effects of Dibutyl and Monobutyl Phthalates on the Steroidogenesis of Rat Immature Leydig Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096864
Modern Language Association (MLA)
Li, Linxi…[et al.]. Comparison of the Effects of Dibutyl and Monobutyl Phthalates on the Steroidogenesis of Rat Immature Leydig Cells. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096864
American Medical Association (AMA)
Li, Linxi& Chen, Xiaomin& Hu, Guo-Xin& Wang, Sicong& Xu, Renai& Zhu, Qiqi…[et al.]. Comparison of the Effects of Dibutyl and Monobutyl Phthalates on the Steroidogenesis of Rat Immature Leydig Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096864
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096864