Expression of miRNA-122 Induced by Liver Toxicants in Zebrafish
Joint Authors
Nam, Hyun-Sik
Hwang, Kyu-Seok
Jeong, Yun-Mi
Ryu, Jeong-Im
Choi, Tae-Young
Bae, Myung-Ae
Son, Woo-Chan
You, Kwan-Hee
Son, Hwa-Young
Kim, Cheol-Hee
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
MicroRNA-122 (miRNA-122), also known as liver-specific miRNA, has recently been shown to be a potent biomarker in response to liver injury in mammals.
The objective of this study was to examine its expression in response to toxicant treatment and acute liver damage, using the zebrafish system as an alternative model organism.
For the hepatotoxicity assay, larval zebrafish were arrayed in 24-well plates.
Adult zebrafish were also tested and arrayed in 200 mL cages.
Animals were exposed to liver toxicants (tamoxifen or acetaminophen) at various doses, and miRNA-122 expression levels were analyzed using qRT-PCR in dissected liver, brain, heart, and intestine, separately.
Our results showed no significant changes in miRNA-122 expression level in tamoxifen-treated larvae; however, miRNA-122 expression was highly induced in tamoxifen-treated adults in a tissue-specific manner.
In addition, we observed a histological change in adult liver (0.5 μM) and cell death in larval liver (5 μM) at different doses of tamoxifen.
These results indicated that miRNA-122 may be utilized as a liver-specific biomarker for acute liver toxicity in zebrafish.
American Psychological Association (APA)
Nam, Hyun-Sik& Hwang, Kyu-Seok& Jeong, Yun-Mi& Ryu, Jeong-Im& Choi, Tae-Young& Bae, Myung-Ae…[et al.]. 2016. Expression of miRNA-122 Induced by Liver Toxicants in Zebrafish. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1096894
Modern Language Association (MLA)
Nam, Hyun-Sik…[et al.]. Expression of miRNA-122 Induced by Liver Toxicants in Zebrafish. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1096894
American Medical Association (AMA)
Nam, Hyun-Sik& Hwang, Kyu-Seok& Jeong, Yun-Mi& Ryu, Jeong-Im& Choi, Tae-Young& Bae, Myung-Ae…[et al.]. Expression of miRNA-122 Induced by Liver Toxicants in Zebrafish. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1096894
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096894