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Biosensor for Hepatocellular Injury Corresponds to Experimental Scoring of Hepatosplenic Schistosomiasis in Mice
Joint Authors
Reisinger, Emil Christian
Sauer, Martin
Sombetzki, Martina
Koslowski, Nicole
Doss, Sandra
Trauner, Michael
Loebermann, Micha
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Severe hepatosplenic injury of mansonian schistosomiasis is caused by Th2 mediated granulomatous response against parasite eggs entrapped within the periportal tissue.
Subsequent fibrotic scarring and deformation/sclerosing of intrahepatic portal veins lead to portal hypertension, ascites, and oesophageal varices.
The murine model of Schistosoma mansoni (S.
mansoni) infection is suitable to establish the severe hepatosplenic injury of disease within a reasonable time scale for the development of novel antifibrotic or anti-infective strategies against S.
mansoni infection.
The drawback of the murine model is that the material prepared for complex analysis of egg burden, granuloma size, hepatic inflammation, and fibrosis is limited due to small amounts of liver tissue and blood samples.
The objective of our study was the implementation of a macroscopic scoring system for mice livers to determine infection-related organ alterations of S.
mansoni infection.
In addition, an in vitro biosensor system based on the detection of hepatocellular injury in HepG2/C3A cells following incubation with serum of moderately (50 S.
mansoni cercariae) and heavily (100 S.
mansoni cercariae) infected mice affirmed the value of our scoring system.
Therefore, our score represents a valuable tool in experimental schistosomiasis to assess severity of hepatosplenic schistosomiasis and reduce animal numbers by saving precious tissue samples.
American Psychological Association (APA)
Sombetzki, Martina& Koslowski, Nicole& Doss, Sandra& Loebermann, Micha& Trauner, Michael& Reisinger, Emil Christian…[et al.]. 2016. Biosensor for Hepatocellular Injury Corresponds to Experimental Scoring of Hepatosplenic Schistosomiasis in Mice. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1096911
Modern Language Association (MLA)
Sombetzki, Martina…[et al.]. Biosensor for Hepatocellular Injury Corresponds to Experimental Scoring of Hepatosplenic Schistosomiasis in Mice. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1096911
American Medical Association (AMA)
Sombetzki, Martina& Koslowski, Nicole& Doss, Sandra& Loebermann, Micha& Trauner, Michael& Reisinger, Emil Christian…[et al.]. Biosensor for Hepatocellular Injury Corresponds to Experimental Scoring of Hepatosplenic Schistosomiasis in Mice. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1096911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096911