![](/images/graphics-bg.png)
A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19
Joint Authors
Bogomolovas, Julius
Bironaitė, Daiva
Bukelskienė, Virginija
Labeit, Siegfried
Šimoliūnas, Egidijus
Rinkūnaitė, Ieva
Smalinskaitė, Luka
Podkopajev, Andrej
Weis, Cleo-Aron
Gretz, Norbert
Grabauskienė, Virginija
Marx, Alexander
Labeit, Dittmar
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Parvovirus B19 (B19V) is a common finding in endomyocardial biopsy specimens from myocarditis and dilated cardiomyopathy patients.
However, current understanding of how B19V is contributing to cardiac damage is rather limited due to the lack of appropriate mice models.
In this work we demonstrate that immunization of BALB/c mice with the major immunogenic determinant of B19V located in the unique sequence of capsid protein VP1 (VP1u) is an adequate model to study B19V associated heart damage.
Methods and Results.
We immunized mice in the experimental group with recombinant VP1u; immunization with cardiac myosin derived peptide served as a positive reference and phosphate buffered saline served as negative control.
Cardiac function and dimensions were followed echocardiographically 69 days after immunization.
Progressive dilatation of left ventricle and decline of ejection fraction were observed in VP1u- and myosin-immunized mice.
Histologically, severe cardiac fibrosis and accumulation of heart failure cells in lungs were observed 69 days after immunization.
Transcriptomic profiling revealed ongoing cardiac remodeling and immune process in VP1u- and myosin-immunized mice.
Conclusions.
Immunization of BALB/c mice with VP1u induces dilated cardiomyopathy in BALB/c mice and it could be used as a model to study clinically relevant B19V associated cardiac damage.
American Psychological Association (APA)
Bogomolovas, Julius& Šimoliūnas, Egidijus& Rinkūnaitė, Ieva& Smalinskaitė, Luka& Podkopajev, Andrej& Bironaitė, Daiva…[et al.]. 2016. A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1096923
Modern Language Association (MLA)
Bogomolovas, Julius…[et al.]. A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1096923
American Medical Association (AMA)
Bogomolovas, Julius& Šimoliūnas, Egidijus& Rinkūnaitė, Ieva& Smalinskaitė, Luka& Podkopajev, Andrej& Bironaitė, Daiva…[et al.]. A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1096923
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096923