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Mitochondria Biogenesis and Bioenergetics Gene Profiles in Isogenic Prostate Cells with Different Malignant Phenotypes
Joint Authors
Burch, Tanya C.
Rhim, Johng S.
Nyalwidhe, Julius O.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-10
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
The most significant hallmarks of cancer are directly or indirectly linked to deregulated mitochondria.
In this study, we sought to profile mitochondria associated genes in isogenic prostate cell lines with different tumorigenic phenotypes from the same patient.
Results.
Two isogenic human prostate cell lines RC77N/E (nonmalignant cells) and RC77T/E (malignant cells) were profiled for expression of mitochondrial biogenesis and energy metabolism genes by qRT-PCR using the Human Mitochondria and the Mitochondrial Energy Metabolism RT2 PCR arrays.
Forty-seven genes were differentially regulated between the two cell lines.
The interaction and regulatory networks of these genes were generated by Ingenuity Pathway Analysis.
UCP2 was the most significantly upregulated gene in primary adenocarcinoma cells in the current study.
The overexpression of UCP2 upon malignant transformation was further validated using human prostatectomy clinical specimens.
Conclusions.
This study demonstrates the overexpression of multiple genes that are involved in mitochondria biogenesis, bioenergetics, and modulation of apoptosis.
These genes may play a role in malignant transformation and disease progression.
The upregulation of some of these genes in clinical samples indicates that some of the differentially transcribed genes could be the potential targets for therapeutic interventions.
American Psychological Association (APA)
Burch, Tanya C.& Rhim, Johng S.& Nyalwidhe, Julius O.. 2016. Mitochondria Biogenesis and Bioenergetics Gene Profiles in Isogenic Prostate Cells with Different Malignant Phenotypes. BioMed Research International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1096967
Modern Language Association (MLA)
Burch, Tanya C.…[et al.]. Mitochondria Biogenesis and Bioenergetics Gene Profiles in Isogenic Prostate Cells with Different Malignant Phenotypes. BioMed Research International No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1096967
American Medical Association (AMA)
Burch, Tanya C.& Rhim, Johng S.& Nyalwidhe, Julius O.. Mitochondria Biogenesis and Bioenergetics Gene Profiles in Isogenic Prostate Cells with Different Malignant Phenotypes. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1096967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096967