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Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley
Joint Authors
Huang, Jianhua
Chen, Zhiwei
Wang, Yifei
Xu, Hongwei
Li, Yingbo
Liu, Chenghong
Lu, Ruiju
Gao, Runhong
Guo, Guimei
He, Ting
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes.
The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease the callus induction.
And the further optimization experiments revealed that the increasing of organic nitrogen sources was much important in callus induction while it seemed different in plant regeneration.
Based on the great effects of organic nitrogen on callus induction, the medium of N6-ANO1/4-2000 might be the best choice for the microspore culture system.
In addition, the phylogenetic analysis indicated that there were clear differences of genetic backgrounds among these barley genotypes, and it also suggested that this medium for microspore culture had widespread utilization in different barley genotypes.
American Psychological Association (APA)
Lu, Ruiju& Chen, Zhiwei& Gao, Runhong& He, Ting& Wang, Yifei& Xu, Hongwei…[et al.]. 2016. Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1096971
Modern Language Association (MLA)
Lu, Ruiju…[et al.]. Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1096971
American Medical Association (AMA)
Lu, Ruiju& Chen, Zhiwei& Gao, Runhong& He, Ting& Wang, Yifei& Xu, Hongwei…[et al.]. Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1096971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096971