Effects of Shaoyao-Gancao Decoction on Infarcted Cerebral Cortical Neurons: Suppression of the Inflammatory Response following Cerebral Ischemia-Reperfusion in a Rat Model
Joint Authors
Zhang, Ying
Jia, Xinling
Yang, Jian
Li, Qing
Yan, Guofeng
Xu, Zhongju
Wang, Jingye
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The mechanisms by which Shaoyao-Gancao decoction (SGD) inhibits the production of inflammatory cytokines in serum and brain tissue after cerebral ischemia-reperfusion (CI-RP) in rats were investigated.
A right middle cerebral artery occlusion was used to induce CI-RP after which the rats were divided into model ( n = 39 ), SGD ( n = 28 ), clopidogrel ( n = 25 ) and sham operated ( n = 34 ) groups.
The Bederson scale was used to evaluate changes in behavioral indices.
The levels of IL-1β, TNF-α, MCP-1, IL-10, RANTES, VEGF, and TGF-β1 in the serum and infarcted brain tissues were measured.
Nissl body and immunohistochemical staining methods were used to detect biochemical changes in neurons, microglial cells, and astrocytes.
Serum levels of VEGF, TNF-α, MCP-1, IL-1β, and IL-10 increased significantly 24 h after CI-RP.
In brain tissue, levels of TNF-α and IL-1β significantly increased 24 h after CI-RP, whereas levels of TGF-β1 and MCP-1 were significantly higher 96 h after CI-RP ( P < 0.05 ).
SGD or clopidogrel after CI-RP reduced TNF-α and IL-1β levels in brain tissue and serum levels of MCP-1, IL-1β, and IL-10.
SGD increased the number of NeuN-positive cells in infarcted brain tissue and reduced the number of IBA1-positive and GFAP-positive cells.
The efficacy of SGD was significantly higher than that of clopidogrel.
American Psychological Association (APA)
Zhang, Ying& Jia, Xinling& Yang, Jian& Li, Qing& Yan, Guofeng& Xu, Zhongju…[et al.]. 2016. Effects of Shaoyao-Gancao Decoction on Infarcted Cerebral Cortical Neurons: Suppression of the Inflammatory Response following Cerebral Ischemia-Reperfusion in a Rat Model. BioMed Research International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1096987
Modern Language Association (MLA)
Zhang, Ying…[et al.]. Effects of Shaoyao-Gancao Decoction on Infarcted Cerebral Cortical Neurons: Suppression of the Inflammatory Response following Cerebral Ischemia-Reperfusion in a Rat Model. BioMed Research International No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1096987
American Medical Association (AMA)
Zhang, Ying& Jia, Xinling& Yang, Jian& Li, Qing& Yan, Guofeng& Xu, Zhongju…[et al.]. Effects of Shaoyao-Gancao Decoction on Infarcted Cerebral Cortical Neurons: Suppression of the Inflammatory Response following Cerebral Ischemia-Reperfusion in a Rat Model. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1096987
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096987