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Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis
Joint Authors
Tony, Hasahya
Junkun, Lu
Erfu, Chu
Xin, Li
Sudeep, K. C.
Mingliang, Zhang
Yanqin, Wang
XiangQian, Qi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aim.
To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity.
Methods.
Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied.
H9c2 cells were cultured with doxorubicin and/or curcumin at various concentrations.
Analysis for apoptosis of H9c2 was done using flow cytometry.
Confocal laser scanning microscopy was used to record the fluorescence intensity ratios and to determine the mitochondrial permeability transition pore (MPTP) opening state.
Oxidative stress was measured using glutathione level, superoxide dismutase activities, and malondialdehyde content.
The effect of doxorubicin and curcumin on PiC gene expression was measured by real-time PCR.
Results.
Curcumin decreased mRNA of PiC and was partly protective against oxidative stress, loss of mitochondrial transmembrane potential, and apoptosis induced by doxorubicin.
Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L.
Conclusion.
Curcumin downregulates PiC and partly protects against doxorubicin induced oxidative stress and myocyte apoptosis.
American Psychological Association (APA)
Junkun, Lu& Erfu, Chu& Tony, Hasahya& Xin, Li& Sudeep, K. C.& Mingliang, Zhang…[et al.]. 2016. Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097010
Modern Language Association (MLA)
Junkun, Lu…[et al.]. Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1097010
American Medical Association (AMA)
Junkun, Lu& Erfu, Chu& Tony, Hasahya& Xin, Li& Sudeep, K. C.& Mingliang, Zhang…[et al.]. Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097010