Methylation Status of SP1 Sites within miR-23a-27a-24-2 Promoter Region Influences Laryngeal Cancer Cell Proliferation and Apoptosis
Joint Authors
Wang, Ye
Zhang, Zhao-Xiong
Chen, Sheng
Qiu, Guang-Bin
Xu, Zhen-Ming
Fu, Wei-Neng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
DNA methylation plays critical roles in regulation of microRNA expression and function.
miR-23a-27a-24-2 cluster has various functions and aberrant expression of the cluster is a common event in many cancers.
However, whether DNA methylation influences the cluster expression and function is not reported.
Here we found a CG-rich region spanning two SP1 sites in the cluster promoter region.
The SP1 sites in the cluster were demethylated and methylated in Hep2 cells and HEK293 cells, respectively.
Meanwhile, the cluster was significantly upregulated and downregulated in Hep2 cells and HEK293 cells, respectively.
The SP1 sites were remethylated and the cluster was significantly downregulated in Hep2 cells into which methyl donor, S-adenosyl-L-methionine, was introduced.
Moreover, S-adenosyl-L-methionine significantly increased Hep2 cell viability and repressed Hep2 cell early apoptosis.
We also found that construct with two SP1 sites had highest luciferase activity and SP1 specifically bound the gene cluster promoter in vitro.
We conclude that demethylated SP1 sites in miR-23a-27a-24-2 cluster upregulate the cluster expression, leading to proliferation promotion and early apoptosis inhibition in laryngeal cancer cells.
American Psychological Association (APA)
Wang, Ye& Zhang, Zhao-Xiong& Chen, Sheng& Qiu, Guang-Bin& Xu, Zhen-Ming& Fu, Wei-Neng. 2016. Methylation Status of SP1 Sites within miR-23a-27a-24-2 Promoter Region Influences Laryngeal Cancer Cell Proliferation and Apoptosis. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097022
Modern Language Association (MLA)
Wang, Ye…[et al.]. Methylation Status of SP1 Sites within miR-23a-27a-24-2 Promoter Region Influences Laryngeal Cancer Cell Proliferation and Apoptosis. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1097022
American Medical Association (AMA)
Wang, Ye& Zhang, Zhao-Xiong& Chen, Sheng& Qiu, Guang-Bin& Xu, Zhen-Ming& Fu, Wei-Neng. Methylation Status of SP1 Sites within miR-23a-27a-24-2 Promoter Region Influences Laryngeal Cancer Cell Proliferation and Apoptosis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097022
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097022