In Vitro and In Vivo Anti-Helicobacter Activities of Eryngium foetidum (Apiaceae)‎, Bidens pilosa (Asteraceae)‎, and Galinsoga ciliata (Asteraceae)‎ against Helicobacter pylori

Abstract EN

This study was performed to evaluate the antimicrobial activities of extracts of Bidens pilosa, Galinsoga ciliata, and Eryngium foetidum against 6 clinical strains of Helicobacter pylori in vitro and in vivo.

Broth microdilution method was used in vitro.

In vivo, Swiss mice were inoculated with H.

pylori and divided into 5 groups; the control group received the vehicle and the four others received 125, 250, and 500 mg/kg of methanol extract of Eryngium foetidum and ciprofloxacin (500 mg/kg) for 7 days, respectively.

Helicobacter pylori colonization and number of colonies in gastric biopsies culture were assessed on days 1 and 7 after treatment.

The lowest MIC value (64 μg/mL) and the best spectrum of bactericidal effect (MBC/MIC = 1) were obtained with the methanol extract of Eryngium foetidum.

The number of H.

pylori infected animals was 17% (plant-extract) and 0% (ciprofloxacin) compared to 100% for the infected untreated group.

Plant-extract ( 381.9 ± 239.5 CFU) and ciprofloxacin ( 248 ± 153.2 CFU) significantly reduced bacterial load in gastric mucosa compared to untreated, inoculated mice ( 14350 ± 690 CFU).

Conclusion.

The present data provided evidence that methanol extract of Eryngium foetidum could be a rich source of metabolites with antimicrobial activity to fight Helicobacter pylori infections.