Effects of Ginger Phenylpropanoids and Quercetin on Nrf2-ARE Pathway in Human BJ Fibroblasts and HaCaT Keratinocytes
Joint Authors
Hajdúch, Marián
Schadich, Ermin
Hlaváč, Jan
Volná, Tereza
Varanasi, Lakshman
Džubák, Petr
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Quercetin and phenylpropanoids are well known chemoprotective compounds identified in many plants.
This study was aimed at determining their effects on activation of Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant response element (Nrf2-ARE) signalling pathway and expression of its important downstream effector phase II detoxification enzyme glutathione-S-transferase P1 (GSTP1) in BJ foreskin fibroblasts and skin HaCaT keratinocytes.
Cell lines and their corresponding Nrf2-ARE luciferase reporter cells were treated by ginger phenylpropanoids and quercetin for 10 h and the level of Nrf2 activity was subsequently determined.
Both, ginger phenylpropanoids and quercetin, significantly increased the level of Nrf2 activity.
Subsequent western blot analyses of proteins showed the increased expression level of glutathione-S-transferase P1 (GSTP1) in BJ cells but not in HaCaT cells.
Such phenomenon of unresponsive downstream target expression in HaCaT cells was consistent with previous studies showing a constitutive expression of their GSTP1.
Thus, while both ginger phenylpropanoids and quercetin have the property of increasing the level of Nrf2 both in HaCaT and in BJ cells, their effects on its downstream signalling were mediated only in BJ cells.
American Psychological Association (APA)
Schadich, Ermin& Hlaváč, Jan& Volná, Tereza& Varanasi, Lakshman& Hajdúch, Marián& Džubák, Petr. 2016. Effects of Ginger Phenylpropanoids and Quercetin on Nrf2-ARE Pathway in Human BJ Fibroblasts and HaCaT Keratinocytes. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097048
Modern Language Association (MLA)
Schadich, Ermin…[et al.]. Effects of Ginger Phenylpropanoids and Quercetin on Nrf2-ARE Pathway in Human BJ Fibroblasts and HaCaT Keratinocytes. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1097048
American Medical Association (AMA)
Schadich, Ermin& Hlaváč, Jan& Volná, Tereza& Varanasi, Lakshman& Hajdúch, Marián& Džubák, Petr. Effects of Ginger Phenylpropanoids and Quercetin on Nrf2-ARE Pathway in Human BJ Fibroblasts and HaCaT Keratinocytes. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097048