Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
Joint Authors
An, Li-Zhe
Li, Ming
Liang, Zhaoxu
Di, Cuixia
Fang, Weikuan
Wu, Kaichao
Chen, Maoshan
He, Shanshan
Zeng, Yuan
Jing, Yan
Liang, Jun
Tan, Fang
Li, Song
Chen, Tuo
Liu, Guangxiu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis.
The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch.
& C.A.
Mey (C.
bungeana) are still unknown.
Results.
In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C.
bungeana.
Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids.
Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains.
Phylogenetic analysis suggested that the protein was from TIP2 subgroup.
Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus.
Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C.
bungeana.
Conclusion.
This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C.
bungeana.
Our findings contribute to understanding the water transports in plants under low temperatures.
American Psychological Association (APA)
Li, Ming& Liang, Zhaoxu& Di, Cuixia& Fang, Weikuan& Wu, Kaichao& Chen, Maoshan…[et al.]. 2016. Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1097082
Modern Language Association (MLA)
Li, Ming…[et al.]. Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1097082
American Medical Association (AMA)
Li, Ming& Liang, Zhaoxu& Di, Cuixia& Fang, Weikuan& Wu, Kaichao& Chen, Maoshan…[et al.]. Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1097082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097082