Organogenesis and Ultrastructural Features of In Vitro Grown Canna indica L.
Joint Authors
Mahmad, Noraini
Taha, Rosna Mat
Ali Ahmed Abdul, Bakrudeen
Wafa, Sharifah Nurashikin
Mohajer, Sadegh
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings.
Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks.
Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%).
Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival.
In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces.
Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar.
Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.
American Psychological Association (APA)
Wafa, Sharifah Nurashikin& Taha, Rosna Mat& Mohajer, Sadegh& Mahmad, Noraini& Ali Ahmed Abdul, Bakrudeen. 2016. Organogenesis and Ultrastructural Features of In Vitro Grown Canna indica L.. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097162
Modern Language Association (MLA)
Wafa, Sharifah Nurashikin…[et al.]. Organogenesis and Ultrastructural Features of In Vitro Grown Canna indica L.. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097162
American Medical Association (AMA)
Wafa, Sharifah Nurashikin& Taha, Rosna Mat& Mohajer, Sadegh& Mahmad, Noraini& Ali Ahmed Abdul, Bakrudeen. Organogenesis and Ultrastructural Features of In Vitro Grown Canna indica L.. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097162