The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana
Joint Authors
Zheng, Luping
Yao, Jinai
Gao, Fangluan
Chen, Lin
Zhang, Chao
Lian, Lingli
Xie, Liyan
Wu, Zujian
Xie, Lianhui
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nucleolar proteins play important roles in plant cytology, growth, and development.
Fibrillarin2 is a nucleolar protein of Nicotiana benthamiana (N.
benthamiana).
Its cDNA was amplified by RT-PCR and inserted into expression vector pEarley101 labeled with yellow fluorescent protein (YFP).
The fusion protein was localized in the nucleolus and Cajal body of leaf epidermal cells of N.
benthamiana.
The N.
benthamiana fibrillarin2 (NbFib2) protein has three functional domains (i.e., glycine and arginine rich domain, RNA-binding domain, and α-helical domain) and a nuclear localization signal (NLS) in C-terminal.
The protein 3D structure analysis predicted that NbFib2 is an α/β protein.
In addition, the virus induced gene silencing (VIGS) approach was used to determine the function of NbFib2.
Our results showed that symptoms including growth retardation, organ deformation, chlorosis, and necrosis appeared in NbFib2-silenced N.
benthamiana.
American Psychological Association (APA)
Zheng, Luping& Yao, Jinai& Gao, Fangluan& Chen, Lin& Zhang, Chao& Lian, Lingli…[et al.]. 2016. The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097168
Modern Language Association (MLA)
Zheng, Luping…[et al.]. The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097168
American Medical Association (AMA)
Zheng, Luping& Yao, Jinai& Gao, Fangluan& Chen, Lin& Zhang, Chao& Lian, Lingli…[et al.]. The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097168
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097168