Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease
Joint Authors
Wu, Chi-Sheng
Lin, Fen-Chiung
Chen, Shu-Jen
Chen, Yung-Lung
Chung, Wen-Jung
Cheng, Cheng-I.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Various microRNAs (miRNAs) are used as markers of acute coronary syndrome, in which heparinization is considered mandatory therapy.
Nevertheless, a standard method of handling plasma samples has not been proposed, and the effects of heparin treatment on miRNA detection are rarely discussed.
Materials and Method.
This study used quantitative polymerase chain reaction (qPCR) analysis to investigate how storage temperature, standby time, hemolysis, and heparin treatment affect miRNA measurement in plasma samples from 25 patients undergoing cardiac catheterization.
Results.
For most miRNAs, the qPCR results remained consistent during the first 2 hours.
The miRNA signals did not significantly differ between samples stored at 4°C before processing and samples stored at room temperature (RT) before processing.
miR-451a/miR-23a ratio < 60 indicated < 0.12% hemolysis with 100% sensitivity and 100% specificity.
Pretreatment with 0.25 U heparinase I recovered qPCR signals that were reduced by in vivo heparinization.
Conclusions.
For miRNA measurement, blood samples stored at RT should be processed into plasma within 2 hours after withdrawal and should be pretreated with 0.25 U heparinase I to overcome heparin-attenuated miRNA signals.
The miR-451a/miR-23a ratio is a reliable indicator of significant hemolysis.
American Psychological Association (APA)
Wu, Chi-Sheng& Lin, Fen-Chiung& Chen, Shu-Jen& Chen, Yung-Lung& Chung, Wen-Jung& Cheng, Cheng-I.. 2016. Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1097186
Modern Language Association (MLA)
Wu, Chi-Sheng…[et al.]. Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1097186
American Medical Association (AMA)
Wu, Chi-Sheng& Lin, Fen-Chiung& Chen, Shu-Jen& Chen, Yung-Lung& Chung, Wen-Jung& Cheng, Cheng-I.. Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1097186
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097186