Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage
Joint Authors
Weng, Guangyang
He, Xin
Ye, YongBin
Xu, XiaoJun
Wang, Jing
Huang, YuXian
Zhang, MingWan
Guo, KunYuan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Acute graft-versus-host disease (aGVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and a major cause of nonrelapse mortality after allo-HSCT.
A conditioning regimen plays a pivotal role in the development of aGVHD.
To provide a platform for studying aGVHD and evaluating the impact of different conditioning regimens, we established a murine aGVHD model that simulates the clinical situation and can be conditioned with Busulfan-Cyclophosphamide (Bu-Cy) and Fludarabine-Busulfan (Flu-Bu).
In our study, BALB/c mice were conditioned with Bu-Cy or Flu-Bu and transplanted with 2×107 bone marrow cells and 2×107 splenocytes from either allogeneic (C57BL/6) or syngeneic (BALB/c) donors.
The allogeneic recipients conditioned with Bu-Cy had shorter survivals (P<0.05), more severe clinical manifestations, and higher hepatic and intestinal pathology scores, associated with increased INF-γ expression and diminished IL-4 expression in serum, compared to allogeneic recipients conditioned with Flu-Bu.
Moreover, higher donor-derived T-cell infiltration and severely impaired B-cell development were seen in the bone marrow of mice, exhibiting aGVHD and conditioned with Flu-Bu.
Our study showed that the conditioning regimen with Bu-Cy resulted in more severe aGVHD while the Flu-Bu regimen was associated with more extensive and long standing bone marrow damage.
American Psychological Association (APA)
He, Xin& Ye, YongBin& Xu, XiaoJun& Wang, Jing& Huang, YuXian& Weng, Guangyang…[et al.]. 2016. Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097227
Modern Language Association (MLA)
He, Xin…[et al.]. Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097227
American Medical Association (AMA)
He, Xin& Ye, YongBin& Xu, XiaoJun& Wang, Jing& Huang, YuXian& Weng, Guangyang…[et al.]. Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097227