Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples
Joint Authors
Chandu, Dilip
Paul, Sudakshina
Parker, Mathew
Dudin, Yelena
King-Sitzes, Jennifer
Perez, Tim
Mittanck, Don W.
Shah, Manali
Glenn, Kevin C.
Piepenburg, Olaf
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Testing for the presence of genetically modified material in seed samples is of critical importance for all stakeholders in the agricultural industry, including growers, seed manufacturers, and regulatory bodies.
While rapid antibody-based testing for the transgenic protein has fulfilled this need in the past, the introduction of new variants of a given transgene demands new diagnostic regimen that allows distinguishing different traits at the nucleic acid level.
Although such molecular tests can be performed by PCR in the laboratory, their requirement for expensive equipment and sophisticated operation have prevented its uptake in point-of-use applications.
A recently developed isothermal DNA amplification technique, recombinase polymerase amplification (RPA), combines simple sample preparation and amplification work-flow procedures with the use of minimal detection equipment in real time.
Here, we report the development of a highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples and present the results of studies applying the method in both laboratory and field-type settings.
American Psychological Association (APA)
Chandu, Dilip& Paul, Sudakshina& Parker, Mathew& Dudin, Yelena& King-Sitzes, Jennifer& Perez, Tim…[et al.]. 2016. Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1097238
Modern Language Association (MLA)
Chandu, Dilip…[et al.]. Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1097238
American Medical Association (AMA)
Chandu, Dilip& Paul, Sudakshina& Parker, Mathew& Dudin, Yelena& King-Sitzes, Jennifer& Perez, Tim…[et al.]. Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1097238
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097238