iTRAQ-Based Proteomics Identification of Serum Biomarkers of Two Chronic Hepatitis B Subtypes Diagnosed by Traditional Chinese Medicine
Joint Authors
Chen, Maoshan
Yang, Jiankun
Yang, Lichao
Li, Baixue
Zhou, Weilong
Zhuang, Zhenhua
Yang, Bin
Feng, Quansheng
Zhong, Sen
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-29, 29 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-29
Country of Publication
Egypt
No. of Pages
29
Main Subjects
Abstract EN
Background.
Chronic infection with hepatitis B virus (HBV) is a leading cause of cirrhosis and hepatocellular carcinoma.
By traditional Chinese medicine (TCM) pattern classification, damp heat stasis in the middle-jiao (DHSM) and liver Qi stagnation and spleen deficiency (LSSD) are two most common subtypes of CHB.
Results.
In this study, we employed iTRAQ proteomics technology to identify potential serum protein biomarkers in 30 LSSD-CHB and 30 DHSM-CHB patients.
Of the total 842 detected proteins, 273 and 345 were differentially expressed in LSSD-CHB and DHSM-CHB patients compared to healthy controls, respectively.
LSSD-CHB and DHSM-CHB shared 142 upregulated and 84 downregulated proteins, of which several proteins have been reported to be candidate biomarkers, including immunoglobulin (Ig) related proteins, complement components, apolipoproteins, heat shock proteins, insulin-like growth factor binding protein, and alpha-2-macroglobulin.
In addition, we identified that proteins might be potential biomarkers to distinguish LSSD-CHB from DHSM-CHB, such as A0A0A0MS51_HUMAN (gelsolin), PON3_HUMAN, Q96K68_HUMAN, and TRPM8_HUMAN that were differentially expressed exclusively in LSSD-CHB patients and A0A087WT59_HUMAN (transthyretin), ITIH1_HUMAN, TSP1_HUMAN, CO5_HUMAN, and ALBU_HUMAN that were differentially expressed specifically in DHSM-CHB patients.
Conclusion.
This is the first time to report serum proteins in CHB subtype patients.
Our findings provide potential biomarkers can be used for LSSD-CHB and DHSM-CHB.
American Psychological Association (APA)
Yang, Jiankun& Yang, Lichao& Li, Baixue& Zhou, Weilong& Zhong, Sen& Zhuang, Zhenhua…[et al.]. 2016. iTRAQ-Based Proteomics Identification of Serum Biomarkers of Two Chronic Hepatitis B Subtypes Diagnosed by Traditional Chinese Medicine. BioMed Research International،Vol. 2016, no. 2016, pp.1-29.
https://search.emarefa.net/detail/BIM-1097331
Modern Language Association (MLA)
Yang, Jiankun…[et al.]. iTRAQ-Based Proteomics Identification of Serum Biomarkers of Two Chronic Hepatitis B Subtypes Diagnosed by Traditional Chinese Medicine. BioMed Research International No. 2016 (2016), pp.1-29.
https://search.emarefa.net/detail/BIM-1097331
American Medical Association (AMA)
Yang, Jiankun& Yang, Lichao& Li, Baixue& Zhou, Weilong& Zhong, Sen& Zhuang, Zhenhua…[et al.]. iTRAQ-Based Proteomics Identification of Serum Biomarkers of Two Chronic Hepatitis B Subtypes Diagnosed by Traditional Chinese Medicine. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-29.
https://search.emarefa.net/detail/BIM-1097331
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097331