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Potential Osteoinductive Effects of Calcitriol on the m-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum
Joint Authors
Yen, Tzung-Hai
Hong, Hsiang-Hsi
Hong, Adrienne
Wang, Yen-Li
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study characterized alveolar periosteum-derived mesenchymal stem cells (P-MSCs) and examined the hypothesis that 1,25-(OH)2D3 (calcitriol) exerts osteoinductive effects on P-MSCs.
The mRNA expressions of alkaline phosphatase (ALP), bone sialoprotein (BSP), core-binding factor alpha-1 (CBFA1), collagen-1 (Col-1), osteocalcin (OCN), and vitamin D3 receptor (VDR) were assessed after incubation with calcitriol for 2 weeks.
Vitamin C as positive control (Vit.
C-p) increased ALP and CBFA1 mRNA expression at both 1 and 2 weeks and increased BSP and Col-1 mRNA expression only at the first week.
A concentration of 10−8 M calcitriol enhanced ALP, CBFA1, Col-1, and OCN mRNA expression at both weeks and BSP mRNA expression at the first week.
Furthermore, 10−7 M calcitriol increased the mRNA expressions of all compounds at both weeks, except that of CBFA1 at the first week.
10−8 M calcitriol and Vit.
C-p enhanced ALP activity at the second and third weeks.
The results revealed that 10−9, 10−8, and 10−7 M calcitriol induced osteoinduction in alveolar P-MSCs by increasing ALP, CBFA1, Col-1, and OCN mRNA expression.
A 10−7 M calcitriol yielded a higher mRNA expression than Vit.
Cp on VDR and OCN mRNA expression at both weeks and on Col-1 mRNA at the second week.
American Psychological Association (APA)
Hong, Hsiang-Hsi& Hong, Adrienne& Yen, Tzung-Hai& Wang, Yen-Li. 2016. Potential Osteoinductive Effects of Calcitriol on the m-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097361
Modern Language Association (MLA)
Hong, Hsiang-Hsi…[et al.]. Potential Osteoinductive Effects of Calcitriol on the m-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097361
American Medical Association (AMA)
Hong, Hsiang-Hsi& Hong, Adrienne& Yen, Tzung-Hai& Wang, Yen-Li. Potential Osteoinductive Effects of Calcitriol on the m-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097361
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097361