Genome-Wide Identification of Long Noncoding RNAs in Human Intervertebral Disc Degeneration by RNA Sequencing

Joint Authors

Zhao, Bo
Lu, Minjuan
Wang, Dong
Li, Haopeng
He, Xijing

Source

BioMed Research International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-12-20

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Long noncoding RNAs (lncRNAs) are emerging as crucial players in a myriad of biological processes.

However, the precise mechanism and functions of most lncRNAs are poorly characterized.

In this study, we presented genome-wide identification of lncRNAs in the patients with intervertebral disc degeneration (IDD) and spinal cord injury (control) using RNA sequencing (RNA-seq).

A total of 124.6 million raw reads were yielded using Hiseq 2500 platform and approximately 88% clean reads could be aligned to human reference genome in both IDD and control groups.

RNA-seq profiling indicated that 1,854 lncRNAs were differentially expressed (log2 fold change ≥ 1 or ≤-1, p<0.05), in which 1,530 could potentially target 6,386 genes via cis-regulatory effects.

Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for these target genes suggested that lncRNAs were involved in diverse pathways, such as lysosome, focal adhesion, and MAPK signaling.

In addition, a competing endogenous RNA (ceRNA) network was constructed for analyzing the function of lncRNAs.

Further, quantitative real time PCR (qRT-PCR) was used to confirm the differentially expressed lncRNAs and ceRNA network.

In conclusion, our results present the first global identification of lncRNAs in IDD and may provide candidate diagnostic biomarkers for IDD treatment.

American Psychological Association (APA)

Zhao, Bo& Lu, Minjuan& Wang, Dong& Li, Haopeng& He, Xijing. 2016. Genome-Wide Identification of Long Noncoding RNAs in Human Intervertebral Disc Degeneration by RNA Sequencing. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097404

Modern Language Association (MLA)

Zhao, Bo…[et al.]. Genome-Wide Identification of Long Noncoding RNAs in Human Intervertebral Disc Degeneration by RNA Sequencing. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1097404

American Medical Association (AMA)

Zhao, Bo& Lu, Minjuan& Wang, Dong& Li, Haopeng& He, Xijing. Genome-Wide Identification of Long Noncoding RNAs in Human Intervertebral Disc Degeneration by RNA Sequencing. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097404

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1097404