Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis

Joint Authors

Wang, Yujiong
Liu, Xiaoming
Li, Xue
Guan, Cuiping
He, Yulong
Zhou, Xuezhang

Source

BioMed Research International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-06-19

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Staphylococcus epidermidis (S.

epidermidis) is an opportunistic pathogen with low pathogenicity and a cause of the repeated outbreak of bovine mastitis in veterinary clinical settings.

In this report, a biofilm model of S.

epidermidis was generated and the minimal inhibitory concentration (MIC) and sub-MIC (SMIC) on bacterial cultures were assessed for the following agents: total alkaloids of Sophora alopecuroides (TASA), ciprofloxacin (CIP), and erythromycin (ERY).

The formation and characteristic parameters of biofilm were analyzed in terms of XTT assay, silver staining, and confocal laser scanning microscope (CLSM).

Results showed that a sub-MIC of TASA could inhibit 50% biofilm of bacterial activity, while 250-fold MIC of CIP and ERY MICs only inhibited 50% and 47% of biofilm formation, respectively.

All three agents could inhibit the biofilm formation at an early stage, but TASA showed a better inhibitory effect on the late stage of biofilm thickening.

A morphological analysis using CLSM further confirmed the destruction of biofilm by these agents.

These results thus suggest that TASA has an inhibitory effect on biofilm formation of clinic S.

epidermidis, which may be a potential agent warranted for further study on the treatment prevention of infection related to S.

epidermidis in veterinary clinic.

American Psychological Association (APA)

Li, Xue& Guan, Cuiping& He, Yulong& Wang, Yujiong& Liu, Xiaoming& Zhou, Xuezhang. 2016. Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097617

Modern Language Association (MLA)

Li, Xue…[et al.]. Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097617

American Medical Association (AMA)

Li, Xue& Guan, Cuiping& He, Yulong& Wang, Yujiong& Liu, Xiaoming& Zhou, Xuezhang. Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097617

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1097617