Method Based on the β-Lactamase PenPC Fluorescent Labeled for β-Lactam Antibiotic Quantification in Human Plasma
Joint Authors
Andresen, Max
Tapia, Pablo
Wong, Kwok-Yin
Leung, Yun-Chung
Wong, Wai-Ting
Chan, Pak-Ho
Andresen-Vasquez, Max
Alegria, Leyla
Silva, Camila
Downey, Patricio
Soto, Dagoberto
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Recently, Wong et al.
have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs).
Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs.
This method showed high precision and specificity and very low interference from clinically relevant samples.
We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.
American Psychological Association (APA)
Andresen, Max& Wong, Kwok-Yin& Leung, Yun-Chung& Wong, Wai-Ting& Chan, Pak-Ho& Andresen-Vasquez, Max…[et al.]. 2016. Method Based on the β-Lactamase PenPC Fluorescent Labeled for β-Lactam Antibiotic Quantification in Human Plasma. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097745
Modern Language Association (MLA)
Andresen, Max…[et al.]. Method Based on the β-Lactamase PenPC Fluorescent Labeled for β-Lactam Antibiotic Quantification in Human Plasma. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1097745
American Medical Association (AMA)
Andresen, Max& Wong, Kwok-Yin& Leung, Yun-Chung& Wong, Wai-Ting& Chan, Pak-Ho& Andresen-Vasquez, Max…[et al.]. Method Based on the β-Lactamase PenPC Fluorescent Labeled for β-Lactam Antibiotic Quantification in Human Plasma. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097745
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097745