Integrated Analysis of DNA Methylation and mRNA Expression Profiles Data to Identify Key Genes in Lung Adenocarcinoma
Joint Authors
Jin, Xiang
Liu, Xingang
Li, Xiaodan
Guan, Yinghui
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
Lung adenocarcinoma (LAC) is the most frequent type of lung cancer and has a high metastatic rate at an early stage.
This study is aimed at identifying LAC-associated genes.
Materials and Methods.
GSE62950 downloaded from Gene Expression Omnibus included a DNA methylation dataset and an mRNA expression profiles dataset, both of which included 28 LAC tissue samples and 28 adjacent normal tissue samples.
The differentially expressed genes (DEGs) were screened by Limma package in R, and their functions were predicted by enrichment analysis using TargetMine online tool.
Then, protein-protein interaction (PPI) network was constructed using STRING and Cytoscape.
Finally, LAC-associated methylation sites were identified by CpGassoc package in R and mapped to the DEGs to obtain LAC-associated DEGs.
Results.
Total 913 DEGs were identified in LAC tissues.
In the PPI networks, MAD2L1, AURKB, CCNB2, CDC20, and WNT3A had higher degrees, and the first four genes might be involved in LAC through interaction.
Total 8856 LAC-associated methylation sites were identified and mapped to the DEGs.
And there were 29 LAC-associated methylation sites located in 27 DEGs (e.g., SH3GL2, BAI3, CDH13, JAM2, MT1A, LHX6, and IGFBP3).
Conclusions.
These key genes might play a role in pathogenesis of LAC.
American Psychological Association (APA)
Jin, Xiang& Liu, Xingang& Li, Xiaodan& Guan, Yinghui. 2016. Integrated Analysis of DNA Methylation and mRNA Expression Profiles Data to Identify Key Genes in Lung Adenocarcinoma. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097753
Modern Language Association (MLA)
Jin, Xiang…[et al.]. Integrated Analysis of DNA Methylation and mRNA Expression Profiles Data to Identify Key Genes in Lung Adenocarcinoma. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097753
American Medical Association (AMA)
Jin, Xiang& Liu, Xingang& Li, Xiaodan& Guan, Yinghui. Integrated Analysis of DNA Methylation and mRNA Expression Profiles Data to Identify Key Genes in Lung Adenocarcinoma. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097753