Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM
Joint Authors
Mustafa, Ibrahim
Al Marwani, Asma
Mamdouh Nasr, Khuloud
Abdulla Kano, Noora
Hadwan, Tameem
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Usually packed red blood cells (pRBCs) require specific conditions in storage procedures to ensure the maximum shelf life of up to 42 days in 2–6°C.
However, molecular and biochemical consequences can affect the stored blood cells; these changes are collectively labeled as storage lesions.
In this study, the effect of prolonged storage was assessed through investigating morphological changes and evaluating oxidative stress.
Samples from leukodepleted pRBC in SAGM stored at 4°C for 42 days were withdrawn aseptically on day 0, day 14, day 28, and day 42.
Morphological changes were observed using scanning electron microscopy and correlated with osmotic fragility and hematocrit.
Oxidative injury was studied through assessing MDA level as a marker for lipid peroxidation.
Osmotic fragility test showed that extended storage time caused increase in the osmotic fragility.
The hematocrit increased by 6.6% from day 0 to day 42.
The last 2 weeks show alteration in the morphology with the appearance of echinocytes and spherocytes.
Storage lesions and morphological alterations appeared to affect RBCs during the storage period.
Further studies should be performed to develop strategies that will aid in the improvement of stored pRBC quality and efficacy.
American Psychological Association (APA)
Mustafa, Ibrahim& Al Marwani, Asma& Mamdouh Nasr, Khuloud& Abdulla Kano, Noora& Hadwan, Tameem. 2016. Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097804
Modern Language Association (MLA)
Mustafa, Ibrahim…[et al.]. Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1097804
American Medical Association (AMA)
Mustafa, Ibrahim& Al Marwani, Asma& Mamdouh Nasr, Khuloud& Abdulla Kano, Noora& Hadwan, Tameem. Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097804
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097804