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The Expression of HMGB1 in Bone Marrow MSCs Is Upregulated by Hypoxia with Regulatory Effects on the Apoptosis and Adhesion
Joint Authors
Tan, Mei-Yun
Zhang, Cai-Dong
Xia, Bo
Guo, Jiang
Fan, Zhong-Wei
Wu, Tian-Hao
Wang, Sen
Liu, Shao-Feng
Deng, Li
Guo, Xing
Huang, Yong-Can
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background and Aims.
Hypoxia regulates the survival of mesenchymal stem cells (MSCs) but the mechanism is unclear.
In hypoxia, the level of high mobility group box 1 (HMGB1) was increased in many cells which may be involved in the regulation of cell biology.
The aim is to determine whether hypoxia affects the expression of HMGB1 in bone marrow MSCs (BM-MSCs) and to investigate the role of HMGB1 in the apoptosis and adhesion.
Methods.
BM-MSCs were exposed to hypoxia (1% O2) and normoxia (20% O2) and the expression of HMGB1 was measured by RT-PCR and western blotting.
The apoptosis and adhesion of BM-MSCs were evaluated after interfered by different concentrations of HMGB1.
Results.
Expression of HMGB1 in BM-MSCs showed a significant upregulation in hypoxia when compared to those in normoxia.
The adhesion of BM-MSCs was increased by HMGB1 in a concentration-dependent manner; the apoptosis effect of HMGB1 depended on its concentrations: HMGB1 at low concentration (50 ng/mL) promoted the apoptosis of BM-MSCs while HMGB1 at high concentration (≥100 ng/mL) reduced this apoptosis.
Conclusions.
Hypoxia enhanced the expression of HMGB1 in BM-MSCs with influences on apoptosis and adhesion and this could have a significant effect on the regenerative potential of MSC-based strategies.
American Psychological Association (APA)
Tan, Mei-Yun& Zhang, Cai-Dong& Xia, Bo& Guo, Jiang& Fan, Zhong-Wei& Wu, Tian-Hao…[et al.]. 2016. The Expression of HMGB1 in Bone Marrow MSCs Is Upregulated by Hypoxia with Regulatory Effects on the Apoptosis and Adhesion. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097822
Modern Language Association (MLA)
Tan, Mei-Yun…[et al.]. The Expression of HMGB1 in Bone Marrow MSCs Is Upregulated by Hypoxia with Regulatory Effects on the Apoptosis and Adhesion. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1097822
American Medical Association (AMA)
Tan, Mei-Yun& Zhang, Cai-Dong& Xia, Bo& Guo, Jiang& Fan, Zhong-Wei& Wu, Tian-Hao…[et al.]. The Expression of HMGB1 in Bone Marrow MSCs Is Upregulated by Hypoxia with Regulatory Effects on the Apoptosis and Adhesion. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097822