Laboratory Prototype of Bioreactor for Oxidation of Toxic D-Lactate Using Yeast Cells Overproducing D-Lactate Cytochrome c Oxidoreductase
Joint Authors
Karkovska, Maria
Gonchar, Mykhailo V.
Smutok, Oleh V.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
D-lactate is a natural component of many fermented foods like yogurts, sour milk, cheeses, and pickles vegetable products.
D-lactate in high concentrations is toxic for children and people with short bowel syndrome and provokes encephalopathy.
These facts convincingly demonstrate a need for effective tools for the D-lactate removal from some food products.
The main idea of investigation is focused on application of recombinant thermotolerant methylotrophic yeast Hansenula polymorpha “tr6,” overproducing D-lactate: cytochrome c oxidoreductase (EC 1.1.2.4, D-lactate cytochrome c oxidoreductase, D-lactate dehydrogenase (cytochrome), DLDH).
In addition to 6-fold overexpression of DLDH under a strong constitutive promoter (prAOX), the strain of H.
polymorpha “tr6” (gcr1 catX/Δcyb2, prAOX_DLDH) is characterized by impairment in glucose repression of AOX promoter, devoid of catalase and L-lactate-cytochrome c oxidoreductase activities.
Overexpression of DLDH coupling with the deletion of L-lactate-cytochrome c oxidoreductase activity opens possibility for usage of the strain as a base for construction of bioreactor for removing D-lactate from fermented products due to oxidation to nontoxic pyruvate.
A laboratory prototype of column-type bioreactor for removing a toxic D-lactate from model solution based on permeabilized cells of the H.
polymorpha “tr6” and alginate gel was constructed and efficiency of this process was tested.
American Psychological Association (APA)
Karkovska, Maria& Smutok, Oleh V.& Gonchar, Mykhailo V.. 2016. Laboratory Prototype of Bioreactor for Oxidation of Toxic D-Lactate Using Yeast Cells Overproducing D-Lactate Cytochrome c Oxidoreductase. BioMed Research International،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1097865
Modern Language Association (MLA)
Karkovska, Maria…[et al.]. Laboratory Prototype of Bioreactor for Oxidation of Toxic D-Lactate Using Yeast Cells Overproducing D-Lactate Cytochrome c Oxidoreductase. BioMed Research International No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1097865
American Medical Association (AMA)
Karkovska, Maria& Smutok, Oleh V.& Gonchar, Mykhailo V.. Laboratory Prototype of Bioreactor for Oxidation of Toxic D-Lactate Using Yeast Cells Overproducing D-Lactate Cytochrome c Oxidoreductase. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1097865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097865