Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico
Joint Authors
Fernando, Bastida-González
Paola, Zárate-Segura
Ramírez-Hernández, Dolores G.
Chavira-Suárez, Erika
Lara-Padilla, Eleazar
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-03
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Nucleoprotein (N) gene from rabies virus (RABV) is a useful sequence target for variant studies.
Several specific RABV variants have been characterized in different mammalian hosts such as skunk, dog, and bats by using anti-nucleocapsid monoclonal antibodies (MAbs) via indirect fluorescent antibody (IFA) test, a technique not available in many laboratories in Mexico.
In the present study, a total of 158 sequences of N gene from RABV were used to design eight pairs of primers (four external and four internal primers), for typing four different RABV variants (dog, skunk, vampire bat, and nonhematophagous bat) which are most common in Mexico.
The results indicate that the primer and the typing variant from the brain samples, submitted to nested and/or real-time PCR, are in agreement in all four singleplex reactions, and the designed primer pairs are an alternative for use in specific variant RABV typing.
American Psychological Association (APA)
Fernando, Bastida-González& Ramírez-Hernández, Dolores G.& Chavira-Suárez, Erika& Lara-Padilla, Eleazar& Paola, Zárate-Segura. 2016. Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico. BioMed Research International،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1097867
Modern Language Association (MLA)
Fernando, Bastida-González…[et al.]. Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico. BioMed Research International No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1097867
American Medical Association (AMA)
Fernando, Bastida-González& Ramírez-Hernández, Dolores G.& Chavira-Suárez, Erika& Lara-Padilla, Eleazar& Paola, Zárate-Segura. Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1097867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097867