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Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion
Joint Authors
Al-Obaidi, J. R.
Dakheel, Khulood Hamid
Abdul Rahim, Raha
Neela, Vasantha Kumari
Hun, Tan Geok
Yusoff, Khatijah
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-18
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms.
The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay.
In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively.
The predominant genotype was spa type t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers.
Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent.
Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment.
PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation.
There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion.
In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested.
American Psychological Association (APA)
Dakheel, Khulood Hamid& Abdul Rahim, Raha& Neela, Vasantha Kumari& Al-Obaidi, J. R.& Hun, Tan Geok& Yusoff, Khatijah. 2016. Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. BioMed Research International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1097877
Modern Language Association (MLA)
Dakheel, Khulood Hamid…[et al.]. Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. BioMed Research International No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1097877
American Medical Association (AMA)
Dakheel, Khulood Hamid& Abdul Rahim, Raha& Neela, Vasantha Kumari& Al-Obaidi, J. R.& Hun, Tan Geok& Yusoff, Khatijah. Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1097877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097877