Serum Amyloid a Promotes Visfatin Expression in Macrophages
Joint Authors
Wang, Shixun
Zhang, Xincai
Tan, Lei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Visfatin has been reported to exert an important role in the development of atherosclerosis.
However, the mechanism that regulated the expression of Visfatin has not been elucidated yet.
This study aimed to investigate the effect of SAA on the regulation of Visfatin, as well as the potential pathway.
After RAW264.7 macrophages and primary monocytes were stimulated with SAA, the mRNA and protein expression of Visfatin was detected with real-time PCR and western blot, respectively.
The concentration of Visfatin in the supernatant was measured with ELISA.
Formyl peptide receptor 2 (FPR2) agonist (WKYMVm) and inhibitor (WRW4), extracellular signal-regulated kinase 1/2 (ERK1/2) inhibitor (PD98059), and peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist (Rosiglitazone) and inhibitor (GW9662) were used to investigate the mechanism of regulation of Visfatin.
The results demonstrated that SAA upregulated Visfatin expression in cultured RAW264.7 macrophages and in the primary monocytes.
WRW4 decreased SAA-induced Visfatin production, while WKYMVm could induce Visfatin expression.
PD98059 reduced SAA-induced Visfatin production.
What is more, GW9662 inhibited SAA-induced Visfatin production, while Rosiglitazone promoted Visfatin expression.
These results demonstrate that SAA upregulates Visfatin expression via a FPR2/ERK1/2/PPAR-γ signaling pathway.
American Psychological Association (APA)
Wang, Shixun& Zhang, Xincai& Tan, Lei. 2016. Serum Amyloid a Promotes Visfatin Expression in Macrophages. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097896
Modern Language Association (MLA)
Wang, Shixun…[et al.]. Serum Amyloid a Promotes Visfatin Expression in Macrophages. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097896
American Medical Association (AMA)
Wang, Shixun& Zhang, Xincai& Tan, Lei. Serum Amyloid a Promotes Visfatin Expression in Macrophages. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097896
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097896