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HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway
Joint Authors
Yan, Youyou
Liu, Bin
Tian, Xin
Zhao, Lei
Song, Xianjing
Liu, Ning
Li, Tianyi
Yan, Bingdi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objectives.
Elevated plasma homocysteine (Hcy) could lead to endothelial dysfunction and is viewed as an independent risk factor for atherosclerosis.
Heat shock protein 27 (HSP27), a small heat shock protein, is reported to exert protective effect against atherosclerosis.
This study aims to investigate the protective effect of HSP27 against Hcy-induced endothelial cell apoptosis in human umbilical vein endothelial cells (HUVECs) and to determine the underlying mechanisms.
Methods.
Apoptosis, reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) of normal or HSP27-overexpressing HUVECs in the presence of Hcy were analyzed by flow cytometry.
The mRNA and protein expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot.
Results.
We found that Hcy could induce cell apoptosis with corresponding decrease of nitric oxide (NO) level, increase of endothelin-1 (ET-1), intracellular adhesion molecule-1 (ICAM-1), vascular cellular adhesion molecule-1 (VCAM-1), and monocyte chemoattractant protein-1 (MCP-1) levels, elevation of ROS, and dissipation of MMP.
In addition, HSP27 could protect the cell against Hcy-induced apoptosis and inhibit the effect of Hcy on HUVECs.
Furthermore, HSP27 could increase the ratio of Bcl-2/Bax and inhibit caspase-3 activity.
Conclusions.
Therefore, we concluded that HSP27 played a protective role against Hcy-induced endothelial apoptosis through modulation of ROS production and the mitochondrial caspase-dependent apoptotic pathway.
American Psychological Association (APA)
Tian, Xin& Zhao, Lei& Song, Xianjing& Yan, Youyou& Liu, Ning& Li, Tianyi…[et al.]. 2016. HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097907
Modern Language Association (MLA)
Tian, Xin…[et al.]. HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097907
American Medical Association (AMA)
Tian, Xin& Zhao, Lei& Song, Xianjing& Yan, Youyou& Liu, Ning& Li, Tianyi…[et al.]. HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097907