RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients

Joint Authors

Yamanaka, Hisashi
Nanke, Yuki
Yago, Toru
Kawamoto, Manabu
Kobashigawa, Tsuyoshi
Kotake, Shigeru

Source

BioMed Research International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-10-16

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Medicine

Abstract EN

Rheumatoid arthritis (RA) appears as inflammation of synovial tissue and joint destruction.

Receptor activator of NF-κB (RANK) is a member of the TNF receptor superfamily and a receptor for the RANK ligand (RANKL).

In this study, we examined the expression of RANKhigh and CCR6 on CD14+ monocytes from patients with RA and healthy volunteers.

Peripheral blood samples were obtained from both the RA patients and the healthy volunteers.

Osteoclastogenesis from monocytes was induced by RANKL and M-CSF in vitro.

To study the expression of RANKhigh and CCR6 on CD14+ monocytes, two-color flow cytometry was performed.

Levels of expression of RANK on monocytes were significantly correlated with the level of osteoclastogenesis in the healthy volunteers.

The expression of RANKhigh on CD14+ monocyte in RA patients without treatment was elevated and that in those receiving treatment was decreased.

In addition, the high-level expression of RANK on CD14+ monocytes was correlated with the high-level expression of CCR6 in healthy volunteers.

Monocytes expressing both RANK and CCR6 differentiate into osteoclasts.

The expression of CD14+RANKhigh in untreated RA patients was elevated.

RANK and CCR6 expressed on monocytes may be novel targets for the regulation of bone resorption in RA and osteoporosis.

American Psychological Association (APA)

Nanke, Yuki& Kobashigawa, Tsuyoshi& Yago, Toru& Kawamoto, Manabu& Yamanaka, Hisashi& Kotake, Shigeru. 2016. RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097930

Modern Language Association (MLA)

Nanke, Yuki…[et al.]. RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1097930

American Medical Association (AMA)

Nanke, Yuki& Kobashigawa, Tsuyoshi& Yago, Toru& Kawamoto, Manabu& Yamanaka, Hisashi& Kotake, Shigeru. RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097930

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1097930