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Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice
Joint Authors
Zheng, Li Wu
Ma, Li
Huang, Hong Zhang
Yu, Ru Qing
Zwahlen, Roger A.
Cai, Wei Xin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence.
The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice.
A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n=8).
A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back.
Validity assessment of the labelled cancer cells’ tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection.
The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells.
Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging.
UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns.
Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.
American Psychological Association (APA)
Cai, Wei Xin& Zheng, Li Wu& Ma, Li& Huang, Hong Zhang& Yu, Ru Qing& Zwahlen, Roger A.. 2016. Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097936
Modern Language Association (MLA)
Cai, Wei Xin…[et al.]. Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1097936
American Medical Association (AMA)
Cai, Wei Xin& Zheng, Li Wu& Ma, Li& Huang, Hong Zhang& Yu, Ru Qing& Zwahlen, Roger A.. Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097936
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097936