A CRISPR-Based Toolbox for Studying T Cell Signal Transduction
Joint Authors
Chi, Shen
Weiss, Arthur
Wang, Haopeng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
CRISPR/Cas9 system is a powerful technology to perform genome editing in a variety of cell types.
To facilitate the application of Cas9 in mapping T cell signaling pathways, we generated a toolbox for large-scale genetic screens in human Jurkat T cells.
The toolbox has three different Jurkat cell lines expressing distinct Cas9 variants, including wild-type Cas9, dCas9-KRAB, and sunCas9.
We demonstrated that the toolbox allows us to rapidly disrupt endogenous gene expression at the DNA level and to efficiently repress or activate gene expression at the transcriptional level.
The toolbox, in combination with multiple currently existing genome-wide sgRNA libraries, will be useful to systematically investigate T cell signal transduction using both loss-of-function and gain-of-function genetic screens.
American Psychological Association (APA)
Chi, Shen& Weiss, Arthur& Wang, Haopeng. 2016. A CRISPR-Based Toolbox for Studying T Cell Signal Transduction. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097981
Modern Language Association (MLA)
Chi, Shen…[et al.]. A CRISPR-Based Toolbox for Studying T Cell Signal Transduction. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097981
American Medical Association (AMA)
Chi, Shen& Weiss, Arthur& Wang, Haopeng. A CRISPR-Based Toolbox for Studying T Cell Signal Transduction. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097981