Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
Joint Authors
Sera, Nobuyuki
Maeda-Mitani, Eriko
Murakami, Koichi
Oishi, Akira
Etoh, Yoshiki
Fujimoto, Shuji
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
QUB11a is used as a locus for variable number of tandem repeats (VNTR) analysis of Mycobacterium tuberculosis Beijing lineage.
However, amplification of QUB11a occasionally produces large fragments (>1,400 bp) that are not easily measured by capillary electrophoresis because of a lack of the typical stutter peak patterns that are used for counting repeat numbers.
IS6110 insertion may complicate VNTR analysis of large QUB11a fragments in M.
tuberculosis.
We established a method for determining both tandem repeat numbers and IS6110 insertion in the QUB11a locus of M.
tuberculosis using capillary electrophoresis analysis and BsmBI digestion.
All 29 large QUB11a fragments (>1,200 bp) investigated contained IS6110 insertions and varied in the number of repeats (18 patterns) and location of IS6110 insertions.
This method allows VNTR analysis with high discrimination.
American Psychological Association (APA)
Maeda-Mitani, Eriko& Murakami, Koichi& Oishi, Akira& Etoh, Yoshiki& Sera, Nobuyuki& Fujimoto, Shuji. 2016. Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1098056
Modern Language Association (MLA)
Maeda-Mitani, Eriko…[et al.]. Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1098056
American Medical Association (AMA)
Maeda-Mitani, Eriko& Murakami, Koichi& Oishi, Akira& Etoh, Yoshiki& Sera, Nobuyuki& Fujimoto, Shuji. Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1098056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098056