Aryl Hydrocarbon Receptor Activation by TCDD Modulates Expression of Extracellular Matrix Remodeling Genes during Experimental Liver Fibrosis
Joint Authors
Lamb, Cheri L.
Cholico, Giovan N.
Perkins, Daniel E.
Fewkes, Michael T.
Oxford, Julia Thom
Lujan, Trevor J.
Morrill, Erica E.
Mitchell, Kristen A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-08
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The aryl hydrocarbon receptor (AhR) is a soluble, ligand-activated transcription factor that mediates the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
Increasing evidence implicates the AhR in regulating extracellular matrix (ECM) homeostasis.
We recently reported that TCDD increased necroinflammation and myofibroblast activation during liver injury elicited by carbon tetrachloride (CCl4).
However, TCDD did not increase collagen deposition or exacerbate fibrosis in CCl4-treated mice, which raises the possibility that TCDD may enhance ECM turnover.
The goal of this study was to determine how TCDD impacts ECM remodeling gene expression in the liver.
Male C57BL/6 mice were treated for 8 weeks with 0.5 mL/kg CCl4, and TCDD (20 μg/kg) was administered during the last two weeks.
Results indicate that TCDD increased mRNA levels of procollagen types I, III, IV, and VI and the collagen processing molecules HSP47 and lysyl oxidase.
TCDD also increased gelatinase activity and mRNA levels of matrix metalloproteinase- (MMP-) 3, MMP-8, MMP-9, and MMP-13.
Furthermore, TCDD modulated expression of genes in the plasminogen activator/plasmin system, which regulates MMP activation, and it also increased TIMP1 gene expression.
These findings support the notion that AhR activation by TCDD dysregulates ECM remodeling gene expression and may facilitate ECM metabolism despite increased liver injury.
American Psychological Association (APA)
Lamb, Cheri L.& Cholico, Giovan N.& Perkins, Daniel E.& Fewkes, Michael T.& Oxford, Julia Thom& Lujan, Trevor J.…[et al.]. 2016. Aryl Hydrocarbon Receptor Activation by TCDD Modulates Expression of Extracellular Matrix Remodeling Genes during Experimental Liver Fibrosis. BioMed Research International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1098082
Modern Language Association (MLA)
Lamb, Cheri L.…[et al.]. Aryl Hydrocarbon Receptor Activation by TCDD Modulates Expression of Extracellular Matrix Remodeling Genes during Experimental Liver Fibrosis. BioMed Research International No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1098082
American Medical Association (AMA)
Lamb, Cheri L.& Cholico, Giovan N.& Perkins, Daniel E.& Fewkes, Michael T.& Oxford, Julia Thom& Lujan, Trevor J.…[et al.]. Aryl Hydrocarbon Receptor Activation by TCDD Modulates Expression of Extracellular Matrix Remodeling Genes during Experimental Liver Fibrosis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1098082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098082