Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells
Joint Authors
Fu, Zhimin
Luo, Bin
Li, Mingpeng
Peng, Bin
Wang, Zheng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We aimed to explore the effects of raloxifene (RAL) on the proliferation and apoptosis of human aortic valve interstitial cells (AVICs).
Different concentrations of RAL were used to act on AVICs.
MTS kit is used to test the effects of different concentrations of RAL on the proliferation of AVICs.
Cell cycle and apoptosis test used flow cytometry after seven-day treatment.
The relative expression levels of caspase-3 and caspase-8 are tested with RT-qPCR and Western blot.
The results of MTS testing revealed that the absorbance value (OD value) of the cells in the concentration groups of 10 and 100 nmol/L RAL at a wavelength of 490 nm at five, seven, and nine days significantly decreased compared with that in the control group.
Meanwhile, the results of flow cytometry of the cells collected after seven days showed that the ratio of the S stage and the cell apoptosis rate of AVICs can be significantly reduced by RAL in the concentration groups of 10 and 100 nmol/L.
The mRNA and protein expressions of caspase-3 and caspase-8 were significantly decreased compared with those in the control group.
This study laid the foundation for further treatment of aortic valve disease by using RAL.
American Psychological Association (APA)
Fu, Zhimin& Luo, Bin& Li, Mingpeng& Peng, Bin& Wang, Zheng. 2016. Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1098138
Modern Language Association (MLA)
Fu, Zhimin…[et al.]. Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1098138
American Medical Association (AMA)
Fu, Zhimin& Luo, Bin& Li, Mingpeng& Peng, Bin& Wang, Zheng. Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1098138
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098138