Secondary Structure and Subunit Composition of Soy Protein In Vitro Digested by Pepsin and Its Relation with Digestibility
Joint Authors
Wang, Zhongjiang
Yang, Yong
Wang, Rui
Sui, Xiaonan
Qi, Baokun
Han, Feifei
Li, Yang
Jiang, Lianzhou
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
In the present study, in vitro digestibility and structure of soybean protein isolates (SPIs) prepared from five soybean varieties were investigated in simulated gastric fluid (SGF), using FT-IR microspectroscopy and SDS-PAGE.
The result indicated that β-conformations were prone to be hydrolyzed by pepsin preferentially and transformed to unordered structure during in vitro digestion, followed by the digestion of α-helix and unordered structure.
A negative linear correlation coefficient was found between the β-conformation contents of five SPIs and their in vitro digestibility values.
The intensities of the protein bands corresponding to 7S and 11S fractions were decreased and many peptide bands appeared at 11~15 kDa during enzymatic hydrolysis.
β-conglycinin was poorly hydrolyzed with pepsin, especially the β-7S subunit.
On the other hand, basic polypeptides of glycinin degraded slower than acidic polypeptides and represented a large proportion of the residual protein after digestion.
11S-A3 of all SPIs disappeared after 1 h digestion.
Moreover, a significant negative linear correlation coefficient (r=-0.89) was found between the β-7S contents of five SPIs and their in vitro digestibility values.
These results are useful for further studies of the functional properties and bioactive properties of these varieties and laid theoretical foundations for the development of the specific functional soy protein isolate.
American Psychological Association (APA)
Yang, Yong& Wang, Zhongjiang& Wang, Rui& Sui, Xiaonan& Qi, Baokun& Han, Feifei…[et al.]. 2016. Secondary Structure and Subunit Composition of Soy Protein In Vitro Digested by Pepsin and Its Relation with Digestibility. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098148
Modern Language Association (MLA)
Yang, Yong…[et al.]. Secondary Structure and Subunit Composition of Soy Protein In Vitro Digested by Pepsin and Its Relation with Digestibility. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1098148
American Medical Association (AMA)
Yang, Yong& Wang, Zhongjiang& Wang, Rui& Sui, Xiaonan& Qi, Baokun& Han, Feifei…[et al.]. Secondary Structure and Subunit Composition of Soy Protein In Vitro Digested by Pepsin and Its Relation with Digestibility. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098148
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098148