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The Effect of Kanamycin and Tetracycline on Growth and Photosynthetic Activity of Two Chlorophyte Algae
Joint Authors
Bashir, Khawaja Muhammad Imran
Cho, Man-Gi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Antibiotics are routinely used in microalgae culture screening, stock culture maintenance, and genetic transformation.
By studying the effect of antibiotics on microalgae growth, we can estimate the least value to inhibit growth of undesired pathogens in algal culture.
We studied the effect of kanamycin and tetracycline on the growth and photosynthetic activity of two chlorophyte microalgae, Dictyosphaerium pulchellum and Micractinium pusillum.
We measured CFU mL−1 on agar plates, optical density, fluorescence yields, and photosynthetic inhibition.
Our results showed a significant effect of kan and tet on the tested microalgae species except tet, which showed a minor effect on M.
pusillum.
Both antibiotics are believed to interact with the protein synthesis machinery; hence, the inhibitory effect of the tested antibiotics was further confirmed by isolation and quantification of the whole cell protein.
A significant reduction in protein quantity was observed at concentrations more than 5 mg L−1, except M.
pusillum, which showed only a slight reduction in protein quantity even at the maximum tested concentration of tet (30 mg L−1).
This study can further aid in aquaculture industry, for the maintenance of the microalgae stock cultures and it can also help the microalgae genetic engineers in the construction of molecular markers.
American Psychological Association (APA)
Bashir, Khawaja Muhammad Imran& Cho, Man-Gi. 2016. The Effect of Kanamycin and Tetracycline on Growth and Photosynthetic Activity of Two Chlorophyte Algae. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098157
Modern Language Association (MLA)
Bashir, Khawaja Muhammad Imran& Cho, Man-Gi. The Effect of Kanamycin and Tetracycline on Growth and Photosynthetic Activity of Two Chlorophyte Algae. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098157
American Medical Association (AMA)
Bashir, Khawaja Muhammad Imran& Cho, Man-Gi. The Effect of Kanamycin and Tetracycline on Growth and Photosynthetic Activity of Two Chlorophyte Algae. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098157