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Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis
Joint Authors
Fang, Wei
Qiao, Luo Shi
Ming, Wu
Jian, Qiu
Feng, Yang Wen
Hua, Gao Hong
Zhou, Xiao Xian
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Rubber tree is a major commercial source of natural rubber.
Latex coagulation is delayed by thiols, which belong to the important type of antioxidants in laticifer submembrane, and is composed of glutathione (GSH), cysteine, and methionine.
The rate-limiting enzyme, γ-ECS, plays an important role in regulating the biosynthesis of glutathione under any environment conditions.
To understand the relation between γ-ECS and thiols and to correlate latex flow with one-time tapping and continuous tapping, we cloned and derived the full length of one γ-ECS from rubber tree latex (Hbγ-ECS1).
According to qPCR analysis, the expression levels of Hbγ-ECS1 were induced by tapping and Ethrel stimulation, and the expression was related to thiols content in the latex.
Continuous tapping induced injury, and the expression of HbγECS1 increased with routine tapping and Ethrel-stimulation tapping (more intensive tapping).
According to expression in long-term flowing latex, the gene was related to the duration of latex flow.
HbγECS1 was expressed in E.
coli Rosetta using pET-sumo as an expression vector and the recombinant enzyme was purified; then we achieved 0.827 U/mg specific activity and about 66 kDa molecular weight.
The present study can help us understand the complex role of Hbγ-ECS in thiols biosynthesis, which is influenced by tapping.
American Psychological Association (APA)
Fang, Wei& Qiao, Luo Shi& Ming, Wu& Jian, Qiu& Feng, Yang Wen& Hua, Gao Hong…[et al.]. 2016. Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098159
Modern Language Association (MLA)
Fang, Wei…[et al.]. Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098159
American Medical Association (AMA)
Fang, Wei& Qiao, Luo Shi& Ming, Wu& Jian, Qiu& Feng, Yang Wen& Hua, Gao Hong…[et al.]. Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098159
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098159