Full-Length cDNA, Prokaryotic Expression, and Antimicrobial Activity of UuHb-F-I from Urechis unicinctus
Joint Authors
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Hemoglobin, which widely exists in all vertebrates and in some invertebrates, is possibly a precursor of antimicrobial peptides (AMPs).
However, AMPs in the hemoglobin of invertebrates have been rarely investigated.
This study is the first to report the full-length cDNA, prokaryotic expression, and antimicrobial activity of UuHb-F-I from Urechis unicinctus.
The full-length cDNA sequence of UuHb-F-I was 780 bp with an open-reading frame of 429 bp encoding 142 amino acids.
MALDI-TOF-MS suggested that the recombinant protein of UuHb-F-I (rUuHb-F-I) yielded a molecular weight of 15,168.01 Da, and its N-terminal amino acid sequence was MGLTGAQIDAIK.
rUuHb-F-I exhibited different antimicrobial activities against microorganisms.
The lowest minimum inhibitory concentration against Micrococcus luteus was 2.78–4.63 μ M.
Our results may help elucidate the immune defense mechanism of U.
unicinctus and may provide insights into new AMPs in drug discovery.
American Psychological Association (APA)
Niu, Rongli& Chen, Xiang. 2016. Full-Length cDNA, Prokaryotic Expression, and Antimicrobial Activity of UuHb-F-I from Urechis unicinctus. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098165
Modern Language Association (MLA)
Niu, Rongli& Chen, Xiang. Full-Length cDNA, Prokaryotic Expression, and Antimicrobial Activity of UuHb-F-I from Urechis unicinctus. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098165
American Medical Association (AMA)
Niu, Rongli& Chen, Xiang. Full-Length cDNA, Prokaryotic Expression, and Antimicrobial Activity of UuHb-F-I from Urechis unicinctus. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098165